1 CAF - secreted Annexin A 1 Induces Prostate Cancer Cells to Gain Stem Cell - like 2 Features 3

Lauren A. Geary1, Kevin A. Nash1, Helty Adisetiyo2, Mengmeng Liang1, Chun-Peng 4 Liao3, Joseph H. Jeong4, Ebrahim Zandi5 and Pradip Roy-Burman1,2,6 5 6 University of Southern California, Keck School of Medicine, Department of Pathology, 7 Los Angeles, CA 90033, USA 8 9 University of Southern California, Keck School of Medicine, Department of 10 Biochemistry and Molecular Biology, Los Angeles, CA 90033, USA 11 12 University of Southern California, Keck School of Medicine, Center for Applied 13 Molecular Medicine, Los Angeles, CA 90033, USA 14 15 Medical College of Wisconsin, Department of Dermatology, Milwaukee, WI 53226, USA 16 17 University of Southern California, Keck School of Medicine, Department of Molecular 18 Microbiology and Immunology, Los Angeles, CA 90033, USA 19 20 6To whom correspondence should be addressed: 21 Pradip Roy-Burman 22 University of Southern California 23 Keck School of Medicine 24 Department of Pathology 25 2011 Zonal Avenue, HMR 210B 26 Los Angeles, CA 90033 27 Tel: 323-442-1184 28 Fax: 323-442-3049 29 [email protected] 30 31 Short title: annexin a1 in prostate cancer stem cell biology 32 33 Conflicts of Interest: The authors declare no conflicts of interest 34 35 Abbreviations: CSC, cancer stem cell; CRPC, castration-resistant prostate cancer; 36 AnxA1, annexin a1; CAF, cancer-associated fibroblasts; NPF, normal prostate 37 fibroblasts; UGSM, urogenital sinus mesenchyme; CM, conditioned medium; EMT, 38 epithelial-to-mesenchymal transition; AS, ammonium sulfate; L (Lin), lineage; S (SCA-1), 39 stem cell antigen-1; C (CD49f), α-6 integrin; PKC, protein kinase C; FPR, formyl peptide 40 receptor 41 42